臨床研究>> 其他> 神經生長因子對人血管內皮細胞增殖作用的研究

 

　　[摘要]

 

      目的：研究神經生長因子(Nerve growth factor, NGF)對人血管內皮細胞(Human vascular endothelial cell, HEVC)增殖作用的影響。

      方法：將NGF作用于體外培養的HEVC304，利用酶聯免疫反應檢測其增殖率，利用免疫組織化學的方法檢測對增殖細胞核抗原(Proliferating cell nuclear antigen, PCNA)的表達和標記指數。

      結果：NGF作用組較對照組其細胞增殖率明顯增加(P<0.001), NGF中和抗體組其細胞增殖率下降明顯(P<0.05)。NGF作用組較對照組能明顯增加HEVC對PCNA的表達和PCNA標記指數（P<0.05）， NGF中和抗體組其PCNA表達和PCNA標記指數明顯減少（P<0.05）。

      結論：NGF能明顯促進HEVC的增殖。
　


　　　　Study of effect of nerve growth factor on the human vascular endothelial cell proliferation

　　　Abstract：   Objective   To investigate the effect of nerve growth factor（NGF）on human vascular endothelial cell.  Methods   The effect of NGF on human umbilica vein vascular endothelial cell were used in the third passage.Endothelial cell proliferative rate was assessed using the enzyme linked immunoassay.The proliferating cell nuclear antigen expression and  proliferating cell nuclear antigen lable index were assessed using the immunnohischemistry.  Results  Compared with the control group，the NGF groups whose endothelial cell proliferative rate were increased significantly（P<0.001）.NGF neutralizing antibody de creased the mitogenic effect of NGF significantly（P<0.05）.NGF also increased PCNA expression and PCNA lable index on HEVC304 .NGF－neutralizing antibody decreaed PCNA expression on NGF－stimulated HEVC304（P<0.05）.  Conclusion  NGF could significantly induced the mitosis of human umbilica vein vascular endothelial cell.